Quantitative Analysis of Serum High Density Lipoprotein by Rocket Immunoelectrophoresis

Low-density-lipoprotein apoprotein B in plasma as measured by radial immunodiffusion and rocket immunoelectrophoresis.

Radial immunodiffusion (RID) and rocket immunoelectrophoresis (RIE) are compared with respect to determination of LDL-bound apo B in plasma. Isolated VLDL could not enter a 15 g/L agarose gel when either technique was used. However, in the presence of plasma proteins, migration of VLDL into agarose was enhanced. Only when plasma samples were kept frozen before the assay was plasma VLDL unable t...

Serum High Density Lipoprotein Cholesterol

Study of Serum Paraoxonase and High Density Lipoprotein Fractions in Diabetes

Objective: Significant alteration in lipid profile and antioxidant system occurs in response to diabetes mellitus (DM). Paraoxonase (PON) is a family of three enzymes PON1, PON2 and PON3 associated with high density lipoprotein (HDL). The HDL in human plasma consists of two main sub-fractions HDL2C and HDL3C. We studied the HDL subclasses and HDL associated enzyme paraoxonase with respect to di...

Allotypy of high density lipoprotein of rabbit serum.

A common antigenic polymorphism of high density lipoprotein (HDL) in rabbit serum is described. The presence or absence of an antigen termed Hl 1 appears to be controlled by autosomal dominant inheritance. The polymorphism should be a useful tool in the study of serum lipoproteins, particularly since genetic polymorphisms within the low density lipoprotein are already known in several species. ...

"Rocket" immunoelectrophoresis assay of vitamin D-binding protein (Gc globulin) in human serum.

suchung mit 51Cr-EDTA im abfallenden Plasmaspiegel. Atompraxis 13,357-360 (1967). 5. Stacy, B. D., and Thorburn, G. D., Chromiuin-51-ethylenediaminetetraacetate for the estimation of gbomerular filtration rate. Science 152, 1076-1077 (1966). 6. Watkin, D. M., Barrows, C. H., Jr., and Chow, B. F., Renal clearance of intravenously administered vitamin B12.Proc. Soc. Exp. Biol. Med. 107, 219-221 (...